Qantas will make it mandatory for all of its 22,000 workers to be fully vaccinated against COVID-19.
Frontline employees — including cabin crew, pilots and airport workers — will need to be fully vaccinated by November 15 and the remainder of employees by March 31.
There will be exemptions for those who are unable to be vaccinated for documented medical reasons, which is expected to be very rare.
"Having a fully vaccinated workforce will safeguard our people against the virus but also protect our customers and the communities we fly to," Qantas chief executive Alan Joyce said.
"It's clear that vaccinations are the only way to end the cycle of lockdowns and border closures and for a lot of Qantas and Jetstar employees that means getting back to work again."
Mr Joyce said, as vaccines have become available, the airline has "strongly encouraged" all of its staff to get vaccinated and was offering paid time off to get it done.
Staff 'concerned' about unvaccinated colleagues
The policy follows consultation with Qantas and Jetstar employees, including a survey sent to its 22,000 workers to seek their views on vaccination.
The company said, of 12,000 responses, 89 per cent had already been vaccinated or are planning to be and only 4 per cent were unwilling or unable to get the jab.
Around three-quarters said it should be a requirement for all employees to be vaccinated and would be concerned if other employees in the workplace were not vaccinated.
"This was one of the largest responses to any survey we've conducted, even with thousands of our people stood down, which shows just how important this is for them," Mr Joyce noted.